Please welcome Amy Jean Porter's horse T-shirt. For the next few days, the shirt is 20 percent off. - - - -

W A R N I N G S   A F F I X E D   T O L A B O R A T O R Y   D O O R S A T   T H E   M A S S A C H U S E T T S I N S T I T U T E   O F   T E C H N O L O G Y . - - - - [As a service to our readers, we will be gathering and reprinting notices posted on laboratory doors at the Massachusetts Institute of Technology. The following notice was found affixed to a door in the main biology building on the campus of MIT. It appears below unaltered. (Note: if you have notices that you have found posted to doors at MIT, please email them to mcsweeneysmail@yahoo.com with "MIT Doors" in the Subject header.)] - - - - E. coli ORFmers E. coli ORFmers are PCR primer pairs that have been designed to amplify each open reading frame (ORF) in E. coli, from the start codon to the stop codon. Genosys Biotechnologies, Inc. offers 4,290 primer pairs, representing all protein-coding genes. The E. coli ORFmers are the direct result of a collaboration with the E. coli Genome Group, headed by Dr. Fred Blattner at the Department of Genetics, University of Wisconsin-Madison (http://www.genetics.wisc.edu/). The PCR primer pairs were designed with the following features: All upstream or "amino-terminal" primers contain an "ATG" start codon (A-primer). All downstream or "carboxyl-terminal" primers contain a "TAA" stop codon (C-primer). A Sap I restriction site has been incorporated into a 5' "adaptamer" sequence in all primers. The "adaptamer" sequence is 13 bases in length. The Sap I enzyme cleaves outside its recognition sequence, generating a PCR product with an 5'-ATG-3' overhang at one end and a 5'-TTA-3' overhang at the other end. This feature has been included to facilitate cloning into a vector with the corresponding sites. A pair of "Universal Adaptamer" PCR primers are available to allow re-amplification of any ORF PCR product. ORFmer Primers are sorted according to gene "Functional Group". Primers from any functional group may be ordered as individual tubes or in a 96-well micro-plate format. There are a total of 23 functional groups.1 E. coli ORFmer Pricing: $20 per PCR primer pair for orders of 1-20 pairs (2,000 pmol of A- and C-primers in individual tubes) $12 per PCR primer pair for orders of 21 or more pairs (2,000 pmol of A- and C-primers in individual tubes). $850 per 96-well plate (2,000 pmol of A- and C-primers combined per well {4000 pmol total}, 96 primer pairs). Please contact your account representative if you are interested in purchasing a complete set of primers in tube or micro-plate formats (45 micro-plates, 4,290 PCR primer pairs). 1. Blattner, F.R., Plunkett, G., Bloch, C.A., Perna, N.T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J.D., Rode, C.K., Mayhew, G.F., Gregor, J., Davis, N.W., Kirkpatrick, H.A., Goeden, M.A., Rose, D.J., Mau, B. and Shao, Y. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1462 (1997).

- - - - MAIN PAGE   |   ARCHIVES   Memories of Amanda Davis   Red dot denotes content that is new today. Black dot denotes newish content. McSWEENEY'S STORE SUBSCRIBE TO: McSWEENEY'S THE BELIEVER WHOLPHIN FUTURE McSWEENEY'S BOOKS THE AMANDA DAVIS HIGHWIRE FICTION AWARD INVITE A McSWEENEY'S AUTHOR TO SPEAK IN YOUR TOWN OR COLLEGE McSWEENEY'S MONTHLY MAILING LIST McSWEENEY'S-RELATED EVENTS AND VARIOUS TOUR DATES ORDER INQUIRIES AND ADDRESS CHANGES SUBMISSION GUIDELINES: FOR BOOKS FOR THE QUARTERLY FOR THE WEBSITE FOR WHOLPHIN McSWEENEY'S INTERNSHIPS CONTACT US - - - - LETTERS TO McSWEENEY'S LISTS McSWEENEY'S PREDICTS McSWEENEY'S RECOMMENDS NEW WHOLPHIN FILM DAN LIEBERT, VERBAL CARTOONIST JOKES BY BRIAN BEATTY REVIEWS OF NEW FOOD DISPATCHES FROM MOSCOW SO YOU WANT TO BE PRESIDENT? DISPATCHES FROM THE ANACOSTIA THE WINNER'S CIRCLE WITH ERIC FEEZELL BEN GREENMAN'S FAKE CELEBRITY MUSICALS DISPATCHES FROM A HUMANITARIAN JOURNALIST DEB OLIN UNFERTH'S SICK OF THE REVOLUTION DISPATCHES FROM IRAQ SHORT IMAGINED MONOLOGUES PHILIP GRAHAM SPENDS A YEAR IN LISBON STAINED TEETH: A COLUMN ABOUT WINE DISPATCHES FROM THE NAPOLEONIC WARS AT THE MET KEVIN DOLGIN TELLS YOU ABOUT PLACES YOU SHOULD GO IN EUROPE SONGS OF ENEMIES AND DESERTS: LIVING WITH THE SUDAN LIBERATION ARMY LAWRENCE WESCHLER'S EVERYTHING THAT RISES: A BOOK OF CONVERGENCES THE CONVERGENCES CONTEST ABOUT WHAT IS THE WHAT ABOUT BOWL OF CHERRIES ABOUT COMEDY BY THE NUMBERS ABOUT JOHN BRANDON'S ARKANSAS ABOUT MICHAEL CHABON'S MAPS AND LEGENDS ABOUT UNDERGROUND AMERICA LETTERS FROM AN EARTH BALL TO, OR CONCERNING, SEAN HANNITY DISPATCHES FROM ADJUNCT FACULTY AT A LARGE STATE UNIVERSITY ADVICE FROM A PERSON WITH A BACHELOR'S DEGREE IN PSYCHOLOGY DISPATCHES FROM THE NBA ENTERTAINMENT LEAGUE JOHN MOE'S POP-SONG CORRESPONDENCES B.R. COHEN'S ANNALS OF SCIENCE INTERVIEWS WITH PEOPLE WHO HAVE INTERESTING OR UNUSUAL JOBS OPEN LETTERS TO PEOPLE OR ENTITIES WHO ARE UNLIKELY TO RESPOND DISPATCHES FROM A PUBLIC LIBRARIAN MICHAEL IAN BLACK IS A VERY FAMOUS CELEBRITY DISPATCHES FROM ROY KESEY, AN AMERICAN GUY MARRIED TO A PERUVIAN DIPLOMAT LIVING IN CHINA STEPHEN ELLIOTT'S POKER REPORT - - - - ADDITIONAL MATERIAL